Neutron diffraction study on the structure of rubredoxin from it Pyrococcus furiosus

Kurihara, Kazuo; Tanaka, Ichiro; Adams, M. W. W.*; Jenney, F. E. Jr.*; Moiseeva, N.*; Bau, R.*; Niimura, Nobuo

Journal of the Physical Society of Japan, Vol.70, Supplement A, p.400 - 402, 2001/05

With the new single-crystal diffractometer BIX-3 at the JRR-3M reactor of JAERI, a single-crystal neutron diffraction analysis of the structure of the small protein rubredoxin from the hyperthermophile Pyrococcus furiosus is currently under way. Data were collected at room temperature up to a resolution of 1.5 intervals in and exposure times ranging from 60 to 77 minutes per frame. The completeness factor of the 1.5-. Included in the refinement are 301 hydrogen atoms and 40 deuterium atoms, and 29 water molecules were also identified. In the present model, the current value for R and R are 24.0 and 26.3 , respectively.